Skip to main content
. Author manuscript; available in PMC: 2022 May 6.
Published in final edited form as: Mol Cell. 2019 Jun 6;75(1):172–183.e9. doi: 10.1016/j.molcel.2019.05.002

Figure 3. The frameshift sequence stimulates frameshifting in multi-RNA translation factories.

Figure 3.

(A) Cells were co-transfected with increasing concentrations of short oligo RNA encoding just the frameshift sequence, together with either the multi-frame reporter (+FSS) or control reporter (−FSS). (B) The percentage of translation sites translating just the 0 frame (green), the 0 and −1 frame (cyan), or just the −1 frame (dark blue) at all oligo concentrations for the +FSS and –FSS reporters. (C) The ratio of the intensity of RNA signals within frameshifting sites to non-frameshifting RNA at all oligo concentrations for the +FSS and −FSS reporters. In all bar graphs, the total number of each type of RNA species is shown above each bar and the error represents S.E.M. between cells.