Fig. 1. Procedure of ATMT. (a) The T-DNA plasmid can be produced by recombinant E. coli. The plasmid is then transformed into Agrobacterium cells. Yellow: T-DNA borders. Red: gene of interest. (b) Acetosyringone induces expression of the vir genes which are located in the bacterial chromosome and in the helper plasmid. Pink: vir genes. (c) The vir genes direct the copy, gene transfer and incorporation of the T-DNA into the target cell.²⁸.