Heterologous expression outcomesa.
| Host organism | Gene source | Gene name | Protein | Biosynthesis product | |
|---|---|---|---|---|---|
| Escherichia coli | Aspergillus flavus | BVMOAFL706, 570, 334, 778 | Type I Baeyer–Villiger monooxygenase | Lactones | 93 |
| Penicillium roqueforti FM164 | fgaOx3Pr3 | Chanoclavine-I aldehyde reductase | Festuclavine (EAS pathway) | 73 | |
| Penicillium roqueforti DSM1079 | fgaDHPr | Festuclavine dehydrogenase | |||
| Penicillium camemberti DSM1233 | fgaDHPca | ||||
| Hebeloma cylindrosporum | hcLAAO4 | l-Amino acid oxidase | α-Keto acids | 94 | |
| Aspergillus oryzae | agl1 | α-1,6-Glucosidase (isomaltase) | Glucose | 95 | |
| Aspergillus niger | agdC | ||||
| Fusarium oxysporum | foagl1 | ||||
| Penicillium herquei | hqlA | Non-ribosomal peptide synthetase | Pyrazine | 96 | |
| Saccharomyces cerevisiae | Aspergillus japonicus | easC_Aj | Catalase | Chanoclavine-I (EAS pathway) | 77 |
| easE_Aj | FAD-dependent oxidoreductase | ||||
| Aspergillus japonicus | easD | Oxidase | Cycloclavine (EAS pathway) | 78 | |
| easA and easG | Reductase | ||||
| easH | Dioxygenase | ||||
| Diverse ascomycete and basidiomycete fungal species | — | 22 BGCs with polyketide synthase or UbiA-type cyclase at its core | — | 68 | |
| Xylaria grammica | frlA | P450 monooxygenase | Statin | 97 | |
| frlB, frlF | Polyketide synthase | ||||
| frlC | Enoyl reductase | ||||
| frlD | Transesterase | ||||
| frlG | Thioesterase | ||||
| frlH | HMG-CoA reductase | ||||
| Aspergillus nidulans | npgA | Phosphopanetheinyltransferase | |||
| Aspergillusnidulans | Aspergillus fumigatus | dmaW | Dimethylallyltryptophan synthase | Chanoclavine-I (EAS pathway) | 80 |
| easF | Methyltransferase | ||||
| easE | Oxidoreductase | ||||
| easC | Catalase | ||||
| Aspergillus terreus | — | 9 nonreducing polyketide synthases | — | 81 | |
| AteafoA | Regulator | Asperfuranone | |||
| AteafoB | Efflux pump | ||||
| AteafoC | Esterase/lipase | ||||
| AteafoD | Salicylate monooxygenase | ||||
| AteafoE | Nonreducing polyketide synthase | ||||
| AteafoF | FAD-dependent oxidase | ||||
| AteafoG | Highly reducing polyketide synthase | ||||
| Baccilussubtilis | Fusarium oxysporum | eysn | Non-ribosomal peptide synthetase | Enniatin | 86 |
| Aspergillus fumugatus | Epichloë sp. Lp1. | easA | Isomerase | Agroclavine (EAS pathway) | 87 |
| easH | Dioxygenase | Lysergic acid (EAS pathway) | |||
| cloA | Clavine oxidase (P450 monooxygenase) | ||||
| Aspergillus oryzae | Monascus pilosus | mokA, B | Polyketide synthase | Monacolin K | 88 |
| mokC | P450 monooxygenase | ||||
| mokD | Oxidoreductase | ||||
| mokE | Dehydrogenase | ||||
| mokF | Transesterase | ||||
| mokG | HMG-CoA reductase | ||||
| mokH | Probable transcription factor | ||||
| mokI | Efflux pump | ||||
| Aspergillus nidulans | tdiA | Non-ribosomal peptide synthase | Terrequinone Q | ||
| tdiB | Indoleprenyltransferase | ||||
| tdiC | Oxidoreductase | ||||
| tdiD | PLP-dependent transaminase | ||||
| tdiE | Unknown protein | ||||
| Aspergillus niger | Trichoderma virens | cbhI | Cellobiohydrolase | Cellobiose | 90 |
| Aspergillus terreus | terA | Non-reducing peptide synthase | Orsellinic acid, 6,7-dihydroxymellein and 4-hydroxy-6-methylpyrone (terrein pathway) | 98 | |
| PamyB:terR | Transcriptional regulator | ||||
| Neurospora crassa | Aspergillus terreus | CAD1 | cis-Aconitic acid decarboxylase | Itaconic acid | 91 |
| E. coli-based cell-free system | Brevibaccilus brevis | grsA and grsB1 | Non-ribosomal peptide synthetase | Gramicidin S | 92 |
a
“—” indicates too much text to fit in the table.