Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun;28(6):808–831. doi: 10.1261/rna.079046.121

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Eisen et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

FIGURE 2. — TE values from neuronal tissues and cultures with and without stimulation. (A) Ranges in TE values acquired for unstimulated neuronal samples in this study. The 1–99 percentile range is printed above the distribution. Values are from adult hippocampus (6342), embryonic hippocampus (7443), adult cortex (5019), and unstimulated cortical culture (n = 5129). Otherwise, this panel is as in Figure 1A. (B) Reproducibility of TE measurements. Shown is the relationship between the TE values for mRNAs from an unstimulated primary cortical culture and those from an analogous culture in which GFP was expressed from a lentiviral vector. (C) Comparison of TE values obtained from different neuronal samples. The heatmap indicates TE values obtained from mRNAs that passed the expression cutoffs in all five of the indicated samples (n = 3347). For each sample, values were z-score normalized and then clustered using k-means. The gene index is plotted as a function of sample and colored by normalized TE (key). (D) Modest effect of stimulation on TE values. Cortical cultures were stimulated with either BDNF (left) or glu/gly (right), and TE values were compared with those observed without stimulation (n = 4705 and 4731, respectively).