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. 2021 Jun 4;118(6):1520–1534. doi: 10.1093/cvr/cvab188

Figure 3.

Figure 3

PGC-1α deficiency suppresses cardiomyocyte energy metabolism. (A–C) Cells isolated from 18-week-old animals were supplemented with different energy substrates, and basal and FCCP induced maximal energy consumption were determined. (A) Averaged extracellular acidification rate (ECAR) traces (left) and analysis of basal and maximal ECAR (right) in cells supplemented with glucose. (B) Averaged oxygen consumption rate (OCR) traces (left) and analysis of basal and maximal OCR (right) in cells supplemented with glucose. (C) Averaged OCR traces (left) and analysis of basal and maximal OCR (right) in cells supplemented with palmitate. (D) Western blot analysis (left) and quantification (right) of 5′ AMP-activated protein kinase (AMPK) in left ventricular tissue from 18-week-old animals. (E) Western blot analysis (left) and quantification (right) of Thr172 phosphorylated AMPK in left ventricular tissue. (F) Ratio of phosphorylated AMPK and total AMPK in left ventricular tissue. In (A–C), male Ctrl n = 5/25 (animals/seahorse wells), male KO n = 5/25, female Ctrl n = 7/35, female KO n = 7/35. In (D–F), n = 4. Statistical significance from hierarchical linear model (A–C) and ANOVA Bonferroni post hoc test (D and E): *P < 0.05, ¤P < 0.01, #P < 0.001.