Figure 4.

Rescue of primary cilia improves ZO-1 expression and junctional arrangement. (A) Immunofluorescent staining (in red) of ZO-1, ZO-2, Catenin α-1, Catenin β-1, Cldn3, and Cx43 on confluent cultures of ORPK and Tg737^orpk/orpk–Ift88* (IFT88*) ECs. Nuclei were stained with DAPI (blue). (B) Quantification of A for fluorescence intensity (top) or junction organization (bottom). Mean ± SD (top) or distribution (%; bottom). Results are obtained from quantification of five images in three independent experiments. (C) Trans-endothelial resistance in ORPK and IFT88* ECs. Mean ± SD. Results are obtained from seven independent experiments. *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001. Comparison of means, medians, and distributions has been performed using unpaired t-test, non-parametric Mann–Whitney U- and chi-square test, respectively. NA = not applicable.