Figure 2.

Whole-body irradiation and chest-shielded irradiation mediated BMT. (A) Recipient mice (CD45.1⁺) are typically subjected to bone marrow reconstitution with donor cells CD45.2⁺ cells after lethal, whole-body irradiation. (B) Flow cytometry analyses of the donor chimaerism of leucocyte subsets (CD45.2⁺; blue) in peripheral blood (PB) and heart 1 month after BMT as described in (A). Nearly all PB leucocyte subsets and large fraction of cardiac immune cells were replaced with donor BM-derived cells. (B) Recipient CD45.1⁺ are subjected to chest-shielded irradiation, followed by reconstitution with CD45.2⁺ bone marrow cells from donor mice. (D) Flow cytometry analyses of the donor chimaerism of leucocyte subsets (CD45.1⁺; orange) in PB and heart 1 month after BMT as described as (C). While a large fraction of PB leucocyte subsets are replaced with donor-derived cells, the replacement of cardiac-resident macrophage by donor-derived cells is considerably less (except for CCR2⁺ population, data not shown). PB, peripheral blood; WBC, white blood cell; Ly6C^hi Mono, Ly6C^high monocyte; Mac, macrophage; Neut, Neutrophil. Data in this figure are from Wang et al.,⁸¹ and it is republished with permission.