Skip to main content
. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Hepatology. 2021 May 24;74(3):1411–1428. doi: 10.1002/hep.31817

Figure 2: Knockdown of SULF2 Decreases CCA Cell Proliferation and Resistance to Cisplatin Induced Apoptosis.

Figure 2:

(A) HuCCT1 scrRNA or HuCCT1 shSULF2 were cultured counted by the Celigo imaging cytometer daily for 3 days. Decreased SULF2 expression was associated with substantially decreased HuCCT1 cell growth (n=10). (B) Western immunoblotting for SULF2 and Ki-67 showed that suppression of SULF2 protein expression was accompanied by decreased cell proliferation marker Ki-67. (C) Statistical analysis of HuCCT1 scrRNA or HuCCT1 shSULF2 apoptosis assay. NS, not significant (n=10). (D) Western immunoblotting showed no difference in the expression of Caspases 3, 8 and 9 between the HuCCT1 scrRNA group and HuCCT1 shSULF2 knockdown group. (E) Statistical analysis of HuCCT1 scrRNA or HuCCT1 shSULF2 apoptosis assays after treatment with cisplatin at concentrations of 1uM, 5uM, and 10uM for 24h or 48h (n=10 each). (F) HuCCT1 scrRNA or HuCCT1 shSULF2 cells were treated with 10uM cisplatin for 48h. Western immunoblotting showed increased cleaved Caspase 8 and cleaved Caspase 3, and decreased pro-caspase 8 and pro-caspase 3 in HuCCT1 shSULF2 cells compared to HuCCT1 scrRNA cells.