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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Hepatology. 2021 May 24;74(3):1411–1428. doi: 10.1002/hep.31817

Figure 3: Expression of SULF2 Promotes CCA Cell Proliferation and Resistance to Cisplatin Induced Apoptosis.

Figure 3:

(A) CCLP1 Vector or CCLP1 SULF2 were cultured for 3 days and counted daily in the Celigo imaging cytometer (n=10). (B) Western immunoblotting for SULF2 and Ki-67 as a marker of cell proliferation showed that forced expression of SULF2 was accompanied by increased cell proliferation. (C) Representative images of apoptosis assays using the Celigo imaging cytometer. CCLP1 Vector or CCLP1 SULF2 without cisplatin treatment are stained by Fluorochrome-labeled Annexin V (green), Propidium Iodide (PI, red), and Hoechst 33342 (blue). The cells were cultured for 48h in 0uM cisplatin (D) Representative images of apoptosis assays using the Celigo imaging cytometer. CCLP1 Vector or CCLP1 SULF2 cells were treated with 10uM cisplatin for 48h. (E) Statistical analysis of CCLP1 Vector or CCLP1 SULF2 apoptosis assays of untreated cells or cells treated with 10uM cisplatin for 48h (n=10).