Skip to main content
. 2022 Feb 22;11(5):e024008. doi: 10.1161/JAHA.121.024008

Figure 3. GSNOR−⁄− mice exhibit nitroso‐redox imbalance and nitrosative stress that is rescued with ascorbate treatment.

Figure 3

In nonpregnant (NP) and pregnant (17.5 d) C57Bl/6J (B6) and GSNOR−⁄− (knockout [KO]) mice, reactive oxygen species, NO and peroxynitrite levels were determined in isolated cardiomyocyte using epifluorescence by 2’,7’dichlorodihydro‐fluoresceine (H2DCF‒DA, 10mM), 4,5‐diaminofluorescein (DAF‐2DA, 10 mmol/L) and dihydrorhodamine 123 (DHR 123, 25 mmol/L), respectively. Ascorbate (AA) studies included both acute (0.1 mmol/L, 0.5 mmol/L, 1 mmol/L) and chronic (AA provided in drinking water from day 0.5) treatments. A and B, ROS generation and levels, and (C) NO and (D) peroxynitrite levels were significantly higher in cardiomyocyte isolated from pregnant GSNOR−⁄− mice. This increase was prevented with acute and chronic AA treatment. E, Superoxide dismutase (SOD) levels were measured in the placenta using lucigenin‐enhanced chemiluminescence. SOD levels were significantly lower in the KO placentas as compared with controls. Results are shown as mean±SEM. N=3–5 animals per experiment. One‐way ANOVA with Newman Keuls post hoc test.B6 indicates C57Bl/6J mice; KO, GSNOR−⁄− mice; NP, nonpregnant; and SNO, S‐nitrosylated.