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. 2022 Feb 22;11(5):e024008. doi: 10.1161/JAHA.121.024008

Figure 4. Preeclampsia phenotype in the mother and litter size is rescued with ascorbate.

Figure 4

Late pregnant (17.5 d) C57Bl/6J (B6) and GSNOR−⁄− (knockout [KO]) mice were examined. N=4–10 mothers per group. A, Hypertension, (B) proteinuria, and (C) urine macroglobulin levels were rescued with ascorbate treatment. Ascorbate treatment increased (D) cardiac output in knockout (KO) mice at late gestation. E, Pup number was improved, whereas (F) fetal weight remained significantly lower in KO mice treated with ascorbate. G, Impaired placental vascularization was rescued with ascorbate treatment in KO placentas. With ascorbate treatment, (H) placental weight was significantly higher in the KO treated animals as compared with B6‐treated animals. Whereas (I) placental efficiency remained significantly lower in treated KO mice as compared with controls. J, K, VEGFR2 and endothelial nitric oxide synthase placental protein levels were significantly increased in KO (Asc) treated animals as compared with nontreated KO animals. Results are shown as mean±SEM. ***P<0.001, **P<0.01, *P<0.05. One‐way or 2‐way ANOVA with Newman Keuls post hoc test and Student’s t test were performed. Asc, ascorbate; B6, C57Bl/6J mice; KO, GSNOR−⁄− mice; and VEGF, vascular endothelial growth factor.