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. 2019 Nov 11;9(63):36608–36614. doi: 10.1039/c9ra06088j

Reduction of carvones using the ERs pQR1907, pQR1445 and NCRa.

graphic file with name c9ra06088j-u2.jpg
ER (2R,5S)-6 (2R,5R)-7
Yield [%] Yield [%]
pQR1907 82 66
pQR1445 81 n.d.
NCR 78 63
a

Substrate (10 mM), purified ER (0.7–0.8 mg mL−1), NADP+ (2.8 mM), G6PDH (20 U), G6PNa (100 mM), in Tris–HCl (50 mM) and DMSO (10%) at pH 7.4, 30 °C, 1 h, 300 rpm. Reactions were performed in triplicate. Yields and ees were determined by GC analysis; n.d. not determined.