Reduction of carvones using the ERs pQR1907, pQR1445 and NCRa.
| ||
|---|---|---|
| ER | (2R,5S)-6 | (2R,5R)-7 |
| Yield [%] | Yield [%] | |
| pQR1907 | 82 | 66 |
| pQR1445 | 81 | n.d. |
| NCR | 78 | 63 |
Substrate (10 mM), purified ER (0.7–0.8 mg mL−1), NADP+ (2.8 mM), G6PDH (20 U), G6PNa (100 mM), in Tris–HCl (50 mM) and DMSO (10%) at pH 7.4, 30 °C, 1 h, 300 rpm. Reactions were performed in triplicate. Yields and ees were determined by GC analysis; n.d. not determined.