Fig. 5. Knockdown of USP14 prevents the PDGF-BB-induced activation of mTOR/P70S6K signaling in HASMCs. (A) The effect of USP14 on mTOR/P70S6K signaling pathway was examined by detecting the expression levels of mTOR, P70S6K, p-mTOR and p-P70S6K using western blot analysis. (B and C) Quantification analysis of p-mTOR/mTOR and p-P70S6K/P70S6K. (D) Co-IP assay verified the overlap between USP14 and PSMD7 proteins. *p < 0.05 vs. control group; #p < 0.05 vs. si-NC + PDGF-BB group.
