Fig. 7. Inhibitors of USP14 and mTOR/P70S6K pathway aggravated the effects of si-USP14 on HASMCs. HASMCs with different transfections were pre-treated with IU1 (5 μmol l−1) or/and rapamycin (10 μmol l−1) for 2 h, followed by stimulation with PDGF-BB (40 ng ml−1) for 24 h. (A and B) The CCK-8 and transwell assays were performed to evaluate cell proliferation and migration, respectively. (C) The expression levels of VSMCs markers including α-SMA, calponin and SM-MHC were measured using western blot analysis. *p < 0.05 vs. control group; #p < 0.05 vs. PDGF-BB group; &p < 0.05 vs. si-USP14+PDGF-BB group.
