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. Author manuscript; available in PMC: 2022 Jun 3.
Published in final edited form as: Nat Methods. 2021 Jun 3;18(6):631–634. doi: 10.1038/s41592-021-01174-8

Fig. 1 |. Method for evaluating whether L1CAM is soluble or associated with EVs in biofluids.

Fig. 1 |

a, Schematic overview of an SEC experiment for evaluating whether L1CAM is associated with EVs. b, L1CAM concentration in SEC fractions after fractionation of EVs derived from iNGN cells expressing L1CAM (top) and after fractionation of soluble recombinant L1CAM protein (bottom). Data represent a single experiment in which two technical replicates were averaged. This experiment was conducted once. c, Simoa quantification of CD9, CD63, CD81, albumin and L1CAM levels in Sepharose 6B 10-ml SEC fractions of CSF (top) and plasma (bottom). Data represent a single experiment in which a single pooled sample was used, and two technical replicates were averaged. This experiment was conducted six times with biofluid samples from different sources, and the results were consistent.