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. Author manuscript; available in PMC: 2022 Jun 3.
Published in final edited form as: Nat Methods. 2021 Jun 3;18(6):631–634. doi: 10.1038/s41592-021-01174-8

Extended Data Fig. 3 |. Mass spectrometry of L1CAM immunocaptured from plasma.

Extended Data Fig. 3 |

Mass spectrometry analysis shows peptides mapping to different parts of the L1CAM protein. Full length sequence of L1CAM displayed with peptides detected by mass spectrometry shown in green. Blue box indicates L1CAM transmembrane domain and red box indicates amino acid sequence encoded by Exon 25. Top: full length recombinant L1CAM protein standard shows peptides matching an isoform which includes Exon 25. Bottom: Mass Spectrometry of L1CAM immunocaptured from human plasma shows peptides matching the cytosolic domain at the C terminus (emphasized with black arrow). This experiment was conducted once.