Skip to main content
. 2022 Mar 24;11(7):e024205. doi: 10.1161/JAHA.121.024205

Figure 7. The effect of tubastatin A (TubA) on cardiac NOD‐like receptor pyrin domain 3 (NLRP3)‐mediated cell pyroptosis and transcription factor EB (TFEB) signaling after cardiac arrest (CA) and resuscitation in swine.

Figure 7

A, Representative immunoblotting and quantification of histone deacetylase 6 (HDAC6), TEFB, NLRP3, cleaved caspase‐1 and gasdermin D (GSDMD). B, The contents of interleukin‐1β (IL‐1β) and interleukin‐18 (IL‐18) measured by ELISA. C, Representative photographs of immunofluorescence staining of TFEB nuclear translocation (scale bar=150 µm, ×200 magnification). A through C, Tubastatin A (4.5 mg/kg) was administered at 5 minutes after resuscitation. The sham, cardiopulmonary resuscitation (CPR) and CPR+tubastatin A groups contained 6 swine at 24 hours after resuscitation, respectively. Myocardial tissue samples were measured at 24 hours after resuscitation. Data were presented as mean±SD. Group comparisons were performed by 1‐way ANOVA with Bonferroni test. *P<0.05 vs sham group, # P<0.05 vs CPR group.