| Column chromatography |
Human α-LA, bovine α-LA, equine lysozyme |
Depends on removing Ca2+ from protein with EDTA bound to column pre-conditioned with OA |
1/∼1–5 for α-LA/OA, 1/∼5–35 for equine lysozyme/OA |
16–18, 33, 35, 37 and 48
|
| Alternative method |
Bovine α-LA |
Depends on partially heated holo protein followed by loading to OA pre-conditioned column |
Not assayed |
11
|
| Heat-induced method |
Camel, human and bovine α-LA, bovine LF, bLG, camel, human and bovine albumin |
Exposure of holo form of protein to heating at 50–60 °C, then add OA directly |
1/∼2.5–10 for α-LA/OA and bLG/OA, 1/15.8 for bovine albumin/OA, 1/12.9 for human albumin/OA, 1/17.9 for camel albumin/OA |
7, 11–13, 19 and 48
|
| Direct mixing method |
α-LA, parvalbumin, bLG |
Direct incubation of protein with OA at change in pH to acid or alkaline environment |
1/4.5 for OA/α-LA, 1/13 for OA/parvalbumin, 1/17 for OA/bLG |
8, 20, 21 and 23–25
|
