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. Author manuscript; available in PMC: 2022 May 6.
Published in final edited form as: Adv Ther (Weinh). 2020 Apr 28;3(7):2000005. doi: 10.1002/adtp.202000005

Figure 4.

Figure 4.

(a) Representative 3D projection of confocal images stacks of DsRed (Red Fluorescent Protein) expressing E. coli DH5α biofilm after 1-hour treatment with Rhodamine Green-tagged colistin (1 mg. L−1) in presence and absence of PNP. The panels are projection at 90° angle turning along X axis. Scale bars are 20 μm. (b) Integrated intensity of Rhodamine Green and DsRed biofilm where 0 μm represents the top layer and ~8 μm the bottom layer. Checkerboard broth microdilution assays between colistin and P7 PNPs against uropathogenic biofilm (c) P. aeruginosa (CD-1006), (d) E. coli (CD-2). Checkerboard data are representative of ≥2 biological replicates. (e) Table showing MBECs (Minimum Biofilm Eradication Concentration) and FICI (Fractional Inhibitory Concentration) scores obtained for PNP-colistin combination against biofilms.