Skip to main content
. Author manuscript; available in PMC: 2022 Aug 15.
Published in final edited form as: Cancer Res. 2022 Feb 15;82(4):648–664. doi: 10.1158/0008-5472.CAN-21-1705

Figure 1. Analysis of LUAD fibroblasts derived from different tumor regions.

Figure 1.

A, Fibroblast expansion workflow of LUAD fresh specimens. B, Phase contrast microscopy images of NFs, TAFs and TCFs. Specimen 2 is displayed here. C, Representative viSNE analysis of NFs, TAFs and TCFs, using α-SMA, FSP1, GFAT2, CD10, p21 and vimentin as clustering markers. Other biological replicates are available in Supplementary Fig. S1C. Venn diagram showing the gene overlap measured by RNAseq (TPM >1 in all biological replicates of each subtype). D, Venn diagram showing pathway overlap (E) and bar graph (F), showing the top exclusive pathways in NFs (n=3), TAFs (n=3) and TCFs (n=3), analyzed with Ingenuity Pathway Analysis software. G, Heatmap showing relative normalized gene expression of top DEGs in NFs, TAFs and TCFs.