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. Author manuscript; available in PMC: 2022 Aug 15.
Published in final edited form as: Cancer Res. 2022 Feb 15;82(4):648–664. doi: 10.1158/0008-5472.CAN-21-1705

Figure 4. Gene expression analysis of NFs, TAFs and TCFs after coculture with HCC827 lung adenocarcinoma cancer cells.

Figure 4.

A, Venn diagram showing the gene overlap between NFs (n=3), TAFs (n=3) and TCFs (n=3) cocultures with HCC827 compared to their monoculture control measured by RNAseq analysis (p < 0.05, fold-change > 0.5). Venn diagram showing pathway overlap (B), heatmap showing the main pathways/biological functions differences analyzed with the IPA software (C) and heatmap showing the fold-change of DEGs in the main glucose metabolic pathways between NFs (n=3), TAFs (n=3) and TCFs (n=3) cocultures with HCC827, (D) and heatmap showcasing the number of overlap genes between DEGs in each condition and within each metabolic pathway (E). Color of heatmap represents -log(p-value) of the gene overlap calculated using a hypergeometric test. The numbers in parentheses show the total number of genes from each MSigDB gene set and tile numbers show the number of DEGs for each condition. See p values at Supplementary Fig. S4G. F, Bar graph showing exclusive pathways related to glycans biosynthesis enriched in TAFs cocultures compared to their monoculture control and analyzed with the IPA software.