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. 2022 May 6;8(18):eabm2545. doi: 10.1126/sciadv.abm2545

Fig. 3. Deleting microglial Gpr56 impairs mid- and late-born PV+ interneurons in the MGE.

Fig. 3.

(A) The experimental design to investigate the PV+ interneuron lineage born at E12.5, the early-stage neurogenesis. (B) Representative images of EdU and PV staining in the SSC of Gpr56 cKO mice and controls. (C) A bar graph showing no significant difference in the number of PV+EdU+ cells between control and Gpr56 cKO mice (P = 0.13); n = 6 in control and n = 7 in cKO. (D and G) The experimental design to evaluate the PV+ interneuron lineage born at E14.5 and E15.5, the mid- and late-stage neurogenesis. (E and H) Representative images of EdU and PV staining in the SSC of Gpr56 cKO mice and controls. (F and I) Bar graphs showing the number of PV+EdU+ cells is significantly decreased in the SSC of microglial Gpr56 cKO mice when EdU was injected at E14.5 or E15.5. [n = 5 and 4 in (D) to (F); n = 6 and 5 in (G) to (I)]. White arrows indicate PV+EdU+ cells. Unpaired t test. *P < 0.05 and **P < 0.01. Data are presented as means ± SEM.