Fig. 3. Sensing performance of the PNA microarray-based fluorometric assay for EGFR mutation detection. (A) PAGE analysis of the RCAPs with different concentrations of EGFR mutation (0–10 nM). (B) Fluorescence emission spectra (λex = 648 nm) of RCAP/F-DP hybrids in the presence of 10 μg mL−1 GO, upon addition of different concentrations of EGFR mutation at (0–10 nM). (C) Plot of the fluorescence intensity at 664 nm against the logarithm of target EGFR mutation concentrations. Error bars reflect four separate measurements.
