Primer sequence for qPCRa.
Gene | Primer sequence (5′–3′) |
---|---|
Actb | F: 5′-CACCCGCGAGCACAGCTTCTT-3′ |
R: 5′-CCACCATCACACCCTGGTGCCT-3′ | |
Acp5 | F: 5′-TGTGGCCATCTTTATGCT-3′ |
R: 5′-GTCATTTCTTTGGGGCTT-3′ | |
Ctsk | F: 5′-GGGAGAAAAACCTGAAGC-3′ |
R: 5′-ATTCTGGGGACTCAGAGC-3′ | |
Ctr | F: 5′-TGGTTGAGGTTGTGCCCA-3′ |
R: 5′-CTCGTGGGTTTGCCTCATC-3′ |
Abbreviations: Actb, actin beta; Acp5, acid phosphatase 5, tartrate resistant; Ctsk, cathepsin K; Ctr, calcitonin receptor; F, forward; R, reverse.