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. 2022 May 2;17:1987–2000. doi: 10.2147/IJN.S363168

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© 2022 Zhang et al.

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Transfection properties of various gene complexes. (A) The relative cell viability of HUVECs incubated with CaP/pEGFP, R(L)-CaP/pEGFP and R(D)-CaP/pEGFP for 24 h. The results of (B) cellular uptake, (C) mean fluorescence intensities and (D) subcellular location of gene complexes with Cy5 labelled peNOS. The scale bar is 20 μm. (E) The quantitative results of (F) CLR of Cy5-peNOS and LysoTracker Green, (F) CLR of Cy5-peNOS and Hoechst 33342 to reflect the localization rate of target genes in endo/lysosomes and nucleus, respectively. (G) The representative images and (H) quantitative results of transfection efficiency of different gene carriers was evaluated after 24 h of transporting pEGFP. The scale bar is 100 μm. mean ± SD, n = 3, *P <0.05, ***P < 0.001, ^###P < 0.001.