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. 2022 Apr 22;11:e73421. doi: 10.7554/eLife.73421

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Chemical compound, drug Choline chloride Sigma-Aldrich C7527
Chemical compound, drug D-(+)-Glucose Sigma-Aldrich G8270
Chemical compound, drug D(+)-Saccharose Roth 4621.1
Chemical compound, drug NaHCO3 Sigma-Aldrich S5761
Chemical compound, drug MgCl2 Sigma-Aldrich 63068
Chemical compound, drug Ascorbic acid Roth 6288.1
Chemical compound, drug Sodium pyruvate Roth 8793.1
Chemical compound, drug KCl Sigma-Aldrich 60130
Chemical compound, drug NaH2PO4 Sigma-Aldrich S0751
Chemical compound, drug CaCl2 Sigma-Aldrich 21097
Chemical compound, drug NaCl Sigma-Aldrich 31434
Chemical compound, drug K-Gluconate Sigma-Aldrich G4500
Chemical compound, drug EGTA Sigma-Aldrich E4378
Chemical compound, drug HEPES Sigma-Aldrich H3375
Chemical compound, drug Na2ATP Sigma-Aldrich A2383
Chemical compound, drug Na3GTP Sigma-Aldrich G8877
Chemical compound, drug Creatine-phosphate Sigma-Aldrich P7936
Chemical compound, drug CsCl Sigma-Aldrich 20966
Chemical compound, drug Sodium creatine phosphate Sigma-Aldrich 27920
Chemical compound, drug Spermine Sigma-Aldrich S4264
Chemical compound, drug Lidocaine N-ethyl bromide (QX-314) Sigma-Aldrich L5783
Chemical compound, drug NaOH Sigma-Aldrich S5881
Chemical compound, drug Clozapine N-oxide  Enzo Life BML-NS-105-0025 5 mg/200 ml
Chemical compound, drug Saccharin Sigma-Aldrich 240931
Chemical compound, drug NASPM Tocris 2766
Chemical compound, drug NGS Sigma-Aldrich AB7481
Chemical compound, drug Triton X-100 Sigma-Aldrich X100
Chemical compound, drug Kynurenic acid Sigma-Aldrich K3375
Chemical compound, drug Picrotoxin Tocris 1128
Chemical compound, drug D-APV Tocris 106
Antibody Oxytocin antibody (guinea pig polyclonal) Synaptic Systems #408004 (1:500)
Antibody cFOS antibody (rabbit monoclonal) Synaptic Systems #226008 (1:5000)
Antibody Neurophysin 2/NP-AVP antibody (mouse monoclonal) Merck Millipore #MABN856 (1:250)
Antibody HA-tag (6E2) antibody (mouse monoclonal) Cell Signaling Technology #2367 (1:200)
Antibody Oxytocin antibody (rabbit polyclonal) Merck Millipore #AB911 (1:500)
Antibody Tyrosine Hydroxylase antibody (mouse monoclonal) Merck Millipore #MAB318 (1:500)
Antibody Goat anti-mouse Alexa Fluor 555 (polyclonal) Thermo Fisher Scientific A21422 (1:500)
Antibody Goat anti-rabbit Alexa Fluor 488 (polyclonal) Thermo Fisher Scientific A11008 (1:500)
Antibody Goat anti-mouse Alexa Fluor 594 (polyclonal) Thermo Fisher Scientific A11005 (1:500)
Antibody Goat anti-mouse Alexa Fluor 647 (polyclonal) Thermo Fisher Scientific A21235 (1:500)
Antibody Goat anti-guinea pig Alexa Fluor 555 (polyclonal) Thermo Fisher Scientific A21435 (1:500)
Other Mounting medium with DAPI Abcam ab104139 Mounting media used for immunofluorescence
Other Alexa Fluor-488 hydrazydes Thermo Fisher Scientific #A10436 10 mM—GFP tracer used for DA neurons validation (Figure 5—figure supplement 2)
Other Cholera Toxin Subunit B, Alexa Fluor 488 Conjugate Thermo Fisher Scientific #C34775 Retrograde tracer used in Figures 3 and 5 and Figure 5—figure supplement 1