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Binding and blocking activities of eight BsAbs determined by ELISA and FACS. (A,B) Dual binding of BsAbs on huPD‐L1‐coating and huTIGIT‐coating ELISA plates. (C,D) Neutralization of PD‐1 binding to PD‐L1 or PVR binding to TIGIT through ELISA. (E,F) BsAbs bound to HEK293‐hPD‐L1 or HEK293‐hTIGIT. (G,H) BsAbs blocked the interaction of HEK293‐hPD‐L1 with hPD‐1 and HEK293‐hTIGIT with hPVR recombinant protein.
