TABLE 1.

KD values and monomer ratios of bispecific antibodies (BsAbs)

	BsAbs	KD (nM)		Monomer ratio
		hPD‐L1	hTIGIT	Native	60°C–1 h	Decrease
Bs#1	T26‐77HL‐wt	7.57	1.79	82.2%	70.5%	11.70%
Bs#2	T26‐77LH‐wt	6.12	2.00	83.3%	79.5%	3.80%
Bs#3	T87‐77HL‐wt	6.02	2.37	81.7%	68.6%	13.10%
Bs#4	T87‐77LH‐wt	6.80	1.89	84.6%	81.1%	3.50%
Bs#5	T26‐77HL‐mu	9.03	4.08	87.2%	84.7%	2.50%
Bs#6	T26‐77LH‐mu	7.80	4.21	96.1%	86.1%	10.00%
Bs#7	T87‐77HL‐mu	7.24	3.30	87.9%	83.7%	4.20%
Bs#8	T87‐77LH‐mu	6.16	2.11	95.5%	87.3%	8.20%

^Notes: :KD values were detected by Octet, and monomer ratios were analysed through SEC‐HPLC.

Abbreviations: HL, VH‐linker‐VL; LH, VL‐linker‐VH; KD, equilibrium dissociation constant; mu, mutant scFv; wt, wild type scFv.