Fig. 2. Dynamic growth profile of Chlorella sp. FC2 in six consecutive batches of cultivation (one control (C), followed by five recycled batches (1–5)). The batch with fresh medium and fresh FC2 cells as the inoculum was considered as the control batch. RWFC represents the batches with the recycling of spent water and fresh FC2 cells as the inoculum for the successive batch. RWHC represents the batches with the recycling of spent water after harvesting and harvested FC2 cells as the inoculum for the successive batch. Harvesting experiments were performed with the microalgal biomass grown in mini-reactors with a working volume of 350 mL. Harvesting experiments were performed with FeSO4 and FeCl3 with the F/X ratios of 2.5 and 3, respectively.
