Fig. 2. Characteristics of recombinant ferritin cages. (a) SDS-PAGE and (b) native-PAGE analyses of recombinant ferritins. Lane M: protein markers and their corresponding molecular masses, lane 1: PcFn, lane 2: PfFn, lane 3: HFn. (c) Negative-stained TEM images of ferritin cages, scale bar is 50 nm. (d) Histograms of size distributions of ferritin cages. (e) DLS analysis of HFn, PfFn, and PcFn nanocages, with diameters in solution of 12.1, 13.3, and 13.9 nm, respectively.
