Fig. 4. Single assay calibration plots and triplex multiplex assay standard curves. Normalized MFI equals to normalized value of target signal. Experimental conditions: (a–c) RNase dilution 1 : 10, chimeric probe concentration 0.01 μM, capture probe coupling concentration 100 pmol/106 microspheres, SAPE dilution 1 : 200 and microspheres density 104 microspheres per well. (d) RNase dilution 3 : 10, each chimeric probe concentration 0.01 μM, each capture probe coupling concentration 100 pmol/106 microspheres, SAPE dilution 3 : 200 and each microspheres density 104 microspheres per well.
