Figure 2.

Spheroid formation model. (A) Model figure depicting the generation of 3D monoculture spheroids in round bottom ultra-low attachment plates. (B) The efficiency of spheroid formation is closely linked to the degree of EMT, and the epithelial cells generated spheroids in monoculture much more efficiently than the mesenchymal cells. In contrast, both epithelial and mesenchymal phenotypes were able to form spheroids when co-cultured with fibroblasts. Figure created with biorender.com.