Table 2.
Antibodies for western blot (WB), Immunocytochemistry with immunofluorescent detection (ICC/IF), and Immunohistochemistry of paraffin sections with immunofluorescent detection (IHC-P/IF).
| Antibody target | Clone | Product number | Supplier* | Dilution | Application* |
|---|---|---|---|---|---|
| CDH1 (E-cadherin) | 4A2 | 14472S | CST | 1:1 000 | WB |
| 1:100 | ICC/IF | ||||
| CDH2 (N-cadherin) | Polyclonal | ab18203 | Abcam | 1:500 | WB |
| VIM (Vimentin) | EPR3776 | ab92547 | Abcam | 1:5 000 | WB |
| 1:100 | ICC/IF | ||||
| TUBA1A (Alpha-tubulin) | DM1A | ab7291 | Abcam | 1:1 000 | ICC/IF |
| EGFRdel (E746-A750del specific) | D6B6 | 2085 | CST | 1:100 | IHC-P/IF |
*CST, Cell Signaling Technology; WB, Western Blot; ICC, Immunocytochemistry; IHC-P, Immunohistochemistry-Paraffin; IF, Immunofluoresence.