Table 3.
List of IMC antibodies.
| Metal tag | Antibody target | Clone | Product number | Supplier | Dilution* |
|---|---|---|---|---|---|
| 141 Pr | ACTA2 (αSMA) | 1A4 | 201505 | Fluidigm | 1:50 |
| 142 Nd | EGFR | D38B1 | 3142013D | Fluidigm | 1:50 |
| 143 Nd | VIM (vimentin) | D21H3 | 201505 | Fluidigm | 1:400 |
| 144 Nd | phospho-Tyr | P-Tyr-100 | 3144024D | Fluidigm | 1:50 |
| 145 Nd | Laminin | polyclonal | PA-16730 | ThermoFisher | 1:500 |
| 148 Nd | Cytokeratin§§ | AE1/AE3 | 3148022D | Fluidigm | 1:200 |
| 150 Nd | MUC1§§ (Mucin1/CD227) | SM3 | 3150032D | Fluidigm | 1:50 |
| 151 Eu | TP63 (p63) | polyclonal | ab53039 | Abcam | 1:50 |
| 154 Sm | HIF1A (HIF-1 α) | EP1215Y | ab210073 | Abcam | 1:50 |
| 155 Gd | GFP | 4B10B2 | MA5-15349 | ThermoFisher | 1:100 |
| 158 Gd | CDH1 (E-cadherin) | 24E10 | 201505 | Fluidigm | 1:100 |
| 159 Tb | RFP | Polyclonal | 600-401-379 | Rockland | 1:50 |
| 161 Dy | MKI67 (Ki67) | B56 | 3161007B | Fluidigm | 1:100 |
| 165 Ho | beta-catenin§§ | D13A1 | 3165032D | Fluidigm | 1:150 |
| 167 Er | MET§§ | D1C2 | 3167020D | Fluidigm | 1:50 |
| 169 Tm | Collagen 1 | Polyclonal | 201505 | Fluidigm | 1:400 |
| 171 Yb | Histone H3§ | D1H2 | 201505 | Fluidigm | 1:4000 |
| 173 Yb | PDGFRB§§ | 28E1 | 3169 | Cell Signaling Technology | 1:50 |
| 175 Lu | pan-actin | D18C11 | 3175032D | Fluidigm | 1:50 |
| 176 Lu | phospho-Histone H3§§ | HTA28 [Ser28] | 3176024D | Fluidigm | 1:100 |
| 191 Ir | Intercalator-Ir | DNA | 201192B | Fluidigm | 250 μM |
| 193 Ir | Intercalator-Ir | DNA | 201192B | Fluidigm | 250 μM |
§Antibodies only included in the first IMC run with untreated samples.
§§Antibodies only included in the second IMC run with the erlotinib and DMSO treated samples.
*Stock concentration for in-house conjugated antibodies is 500 µg/ml.