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Nephron segment markers in organoids
| Segment | Morphology | Location | Immunofluorescence Markers |
|||||
|---|---|---|---|---|---|---|---|---|
| NPHS1 | SYNPO | PODXL | CUBN | LTL | CDH1 | |||
| Podocyte | Bulbous | Periphery | + | + | + | − | − | − |
| Proximal tubule | Straight tubular | Middle | − | − | +/− | + | + | +/− |
| Distal tubule | Branching tubular | Center | − | − | − | − | +/− | + |
NPHS1, nephrin; SYNPO, synaptopodin; PODXL, podocalyxin; CUBN, cubilin; LTL, Lotus tetragonolobus lectin; CDH1, E-cadherin.
