Figure 1.

Experimental design. Piglets were weaned from their dam either at 15 days of age (early weaned, EW) or at 28 days of age (late weaned, LW). Three independent animal cohort were used. At 70 days of age (cohort 1; n = 16 pigs and cohort 2; n = 16 pigs), blood and tissue (jejunum, ileum, and retroperitoneal adipose tissue) samples were harvested. Jejunal and ileal mucosa were prepared for Ussing chamber analyses for intestinal glucose transport experiments. Cohort 3 (n = 12 pigs) was euthanized at 140 days of age for blood and tissue sampling (jejunum and ileum). Image created with BioRender and published with permission.