Figure 2.

Early weaning induces a development shift in small intestinal epithelial SGLT1 and GLUT2 transport. Jejunal and ileal mucosa obtained from 70-day-old female pigs that were previously weaned from their dam at 28 days of age (late wean; blue) or 15 days of age (early wean; orange) were mounted on Ussing chambers. The peak change in transepithelial I_sc (ΔI_sc) was calculated following addition of mucosal d-glucose (10 mM) in jejunum (A) and ileum (E). Representative I_sc tracings of jejunum (B) and ileum (F) after addition of glucose (indicated by red arrow). GLUT2-mediated transport was measured in the jejunum (C) and ileum (G) as determined by NBD-glucose flux across in the presence of the SGLT1 inhibitor phlorizin. Basal transepithelial electrical resistance (TER) was measured in jejunum (D) and ileum (H). Values represent means ± SE for n = 8–16 pigs/weaning age group. Glucose-induced I_sc was performed on two independent cohorts of animals (cohort 1; n = 16 pigs and cohort 2; n = 16 pigs), and data from both cohorts is combined within respective wean age groups. NBDG flux and TER measurements reflect one cohort (cohort 2) of animals. **P < 0.01, **** P < 0.0001, LW vs. EW, Student’s t test. EW, early wean; GLUT2, glucose transporter 2; I_sc, short-circuit current; LW, late wean; SGLT1, sodium-glucose-linked transporter 1. Image created with BioRender and published with permission.