Figure 4.

Early weaning induces a long-term shift in SGLT1 and facilitated GLUT2 and GLUT5 transporters in small intestinal brush-border membranes. Jejunal brush-border membrane vesicles (BBMVs) were isolated from the female pigs that were previously weaned from their dam on day 28 (late wean) or 15 days (early wean) of age for SGLT1, GLUT2, and GLUT5 protein expression via Western blot analysis. Expression level of glucose transporters was determined using densitometric analysis and relative to total blot protein, as described in the methods. Values in A–C (70-day-old pigs) represent the combined data from two independent experimental cohorts (n = 16 pigs/cohort), where the same response was observed. Values in D–F (140-day-old pigs) represent one experimental cohort of pigs (n = 16). Representative Western blot images: molecular weight markers from each blot were from the same blot as protein bands, but not adjacent to the lanes shown (indicated by the line borders). *P < 0.05, **P < 0.01, Student’s t test. GLUT5, fructose transporter; GLUT2, glucose transporter 2; SGLT1, sodium-glucose-linked transporter 1.