Figure 6.
Differential regulation of SGLT1- and GLUT2-mediated glucose transport by β-adrenergic receptors in late-weaned and early-weaned pigs. Ileal mucosa tissues from EW and LW pigs at day 70 of age were mounted on Ussing chamber. Tissues from each pig were pretreated with Ringer (control, con), propranolol (pro), TTX, or Pro + TTX for 20 min before luminal addition of 100 mM d-glucose mixed with NBD-glucose (NBDG). Blockade effect of treated tissues were presented relative to the controls within each pig. A: SGLT1 activity was measured as glucose-induced ΔIsc. B: GLUT2-mediated transport was determined by NBD-G flux in the presence of the SGLT1 inhibitor phlorizin. Values represent means ± SE for n = 8 pigs/weaning age group from one experimental cohort of pigs (cohort 2). *P < 0.05, **P < 0.01, LW vs. EW, two-way ANOVA. EW, early weaned; GLUT2, glucose transporter 2; LW, late weaned; SGLT1, sodium-glucose-linked transporter 1; TTX, tetrodotoxin.