Figure 7.

Differential regulation of SGLT1- and GLUT2-mediated BBM localization by β-adrenergic receptors in late-weaned and early-weaned pigs. Brush-border membrane vesicles (BBMVs) were isolated ileal mucosa mounted on Ussing chamber tissues that were previously treated with propranolol (pro) and tetrodotoxin (TTX) described in Fig. 6 and in methods. Protein abundance (relative to total protein on blot) of SGLT1 (A) and GLUT2 (B) in BBM fractions were quantified via Western blot analysis and densitometric analysis. Effects of Pro and TTX presented as relative densitometry values compared with controls within each pig. *P < 0.05, ***P < 0.001, ****P < 0.0001, LW vs. EW, two-way ANOVA. BBM, brush-border membrane; EW, early weaned; GLUT2, glucose transporter 2; LW, late weaned; SGLT1, sodium-glucose-linked transporter 1.