Fig. 2. Feasibility of P-HP mediated S-EXPAR assay. (A) Fluorescence spectra without (curve a) and with (curve b) target DNA under the same experimental conditions. (B) The nPAGE (12%) analysis. Lane M, 20 bp DNA marker; lane 1, T; lane 2, P-HP; lane 3, P-HP + T; lane 4, P-HP + T + KF polymerase; lane 5, P-HP + KF polymerase + Nb.BbvCI; lane 6: P-HP + T + KF polymerase + Nb.BbvCI. Concentrations of P-HP and T were 1 μM in lanes 1–3, and the concentrations of T, P-HP, and dNTPs in lanes 4–6 were 0.5 μM, 0.5 μM and 500 μM, respectively. The amount of KF polymerase and Nb.BbvCI was 0.08 U and 3 U, respectively.