Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 5;25(5):588–595. doi: 10.1038/s41593-022-01061-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a, Expression of tyrosine hydroxylase (Th) and Nr4a2 in a published scRNA-seq study of mouse midbrain(21). b, Representative FANS plot for enriching midbrain dopaminergic neurons. APC-A (x-axis) represents NR4A2 antibody channel and Brilliant Violet (y-axis) represents DAPI channel. The NR4A2 gate was thresholded to select the top 2.5–4% of all nuclei (red box). c,d, Number of genes (C) and UMIs (unique molecular indicators) (D) per nucleus grouped by donor (n = 11,577 nuclei for subject 3298; 49,759 nuclei from 3322; 29,230 nuclei for 3345; 23,747 nuclei for subject 3346; 24,856 nuclei for subject 3482; 25,243 nuclei for subject 4956; 6,967 for subject 5610; and 13,294 nuclei for subject 6173). e-f, log₁₀ number of genes (nGene) (E) and log₁₀ number of UMIs (nUMI) (F) per cell type (n = 15,684 DA neuronal nuclei; 46,860 non-DA neurons; 5,866 OPC nuclei; 14,579 astrocyte nuclei; 76,837 oligodendrocyte nuclei; 16,755 microglia/macrophage nuclei; and 8,092 endothelial cell/fibroblast nuclei). For all box plots, center bar indicates median value and lower and upper hinges correspond to first and third quartiles respectively. Whisker distance from upper and lower hinges represents ≤1.5× !QR. DA, dopaminergic; non-DA, non-dopaminergic, OPC, oligodendrocyte precursor cell; Olig, oligodendrocyte; Endo/pericyte, endothelial cells/pericytes; MG, microglia/macrophage. g,h, UMAP representations of the NR4A2 + (G) and NR4A2- (H) gated nuclei, colored by cluster. i,j, Violin plots of marker genes for the seven major cell classes in the NR4A2 + (I) and NR4A2- (J) gated nuclei. DA, dopaminergic; non-DA, non-dopaminergic; Astro, astrocyte; MG, microglia/macrophages; OPC, oligodendrocyte precursor cell; Olig, oligodendrocyte; Endo, endothelial cells.