Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 2;25(5):596–606. doi: 10.1038/s41593-022-01052-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a, Representative transmission electron micrographs from corpus callosum body of 6-month-old mice. Scale bar, 2 μm. b, g-ratios from 6-month-old Scn8a^+/mut and Scn8a^+/mut OPC cKO mice. Each dot represents the g-ratio for one axon. c, Mean g-ratios for each mouse. Scn8a^+/+, n = 4 mice; Scn8a^+/+ OPC cKO, n = 4 mice; Scn8a^+/mut, n = 4 mice; Scn8a^+/mut OPC cKO, n = 4 mice. One-way ANOVA, F_3,12 = 8.753, P = 0.0024. Post hoc Tukey’s test: Scn8a^+/mut versus Scn8a^+/+, P = 0.0085, Scn8a^+/mut versus Scn8a^+/+ OPC cKO, P = 0.0042. Scn8a^+/mut OPC cKO mice versus Scn8a^+/+, P > 0.99. Scn8a^+/mut OPC cKO mice versus Scn8a^+/+ OPC cKO, P > 0.99. Scn8a^+/mut OPC cKO versus Scn8a^+/mut mice, P = 0.0066. Scn8a^+/+ versus Scn8a^+/+ OPC cKO, P = 0.9756. d, Representative seizure in a Scn8a^+/mut mouse. e, Continuous EEG recordings showing decreased incidence of seizures (arrowheads) in Scn8a^+/mut OPC cKO mice at 6 months. f, Mean seizures per hour for each mouse. 3 months: Scn8a^+/+, n = 3 mice; Scn8a^+/+ OPC cKO, n = 8 mice; Scn8a^+/mut, n = 7, Scn8a^+/mut OPC cKO, n = 3 mice. One-way ANOVA: F_3,17 = 5.814, P = 0.0063. Post hoc Tukey’s test: Scn8a^+/+ versus Scn8a^+/mut, P = 0.045. Scn8a^+/mut versus Scn8a^+/mut OPC cKO, P = 0.6. 4 months: Scn8a^+/+, n = 5 mice; Scn8a^+/+ OPC cKO, n = 6 mice; Scn8a^+/mut, n = 6 mice, Scn8a^+/mut OPC cKO, n = 4 mice. One-way ANOVA: F_3,17 = 23.05, P < 0.0001. Tukey’s test: Scn8a^+/+ versus Scn8a^+/mut, P < 0.0001. Scn8a^+/mut versus Scn8a^+/mut OPC cKO, P = 0.0193. 6 months: Scn8a^+/+, n = 5 mice; Scn8a^+/+ OPC cKO, n = 5 mice; Scn8a^+/mut, n = 5 mice; Scn8a^+/mut OPC cKO, n = 4 mice. One-way ANOVA: F_3,15 = 11.13, P = 0.0004. Tukey’s test: Scn8a^+/+ versus Scn8a^+/mut, P = 0.0008. Scn8a^+/mut versus Scn8a^+/mut OPC cKO, P = 0.0218. g, Schematic of recording electrodes over somatosensory cortices, created with BioRender. h, Representative seizure from a 6-month-old Scn8a^+/mut mouse with coherence plot. i, Ictal theta band coherence. Scn8a^+/mut, n = 6 mice, Scn8a^+/mut OPC cKO, n = 7 mice. Two-tailed t-test: P = 0.047. Each dot represents the mean for one animal (c, f, i), with group means ± s.e.m. Scn8a^+/+, black dots; Scn8a^+/+ OPC cKO, gray dots; Scn8a^+/mut, red dots; Scn8a^+/mut OPC cKO, blue dots. *P < 0.05, **P < 0.01, ***P < 0.001, NS, non-significant (P > 0.05).

Source data