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Sites of fluorophore incorporation within fluorescent Stop-POST5 complexes.
| Stop-POST5 | Lys within FKVRN | tRNAGln | Ribosomea |
|---|---|---|---|
| Atto(pep) | Atto647 | unlabeled | unlabeled |
| Atto(pep)-Cy(tRNA) | Atto647 | Cy3 | unlabeled |
| Atto(rbsm)-Cy(tRNA) | unlabeled | Cy3 | Atto647N |
| Atto(pep)-Cy(rbsm) | Atto647 | unlabeled | Cy3 |
aribosomes were labeled to a total stoichiometry of ~1/ribosome spread over Lys residues within r-proteins
