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. 2022 May 6;13:2515. doi: 10.1038/s41467-022-30247-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Experimental strategy for the isolation and assessment of self-renewability and differentiation potential of FoxA2+ cells in vivo. B Representative images of colonies derived from FoxA2+ cells isolated from primary (a) and secondary transplants (b). Scale bars, 1 cm. DiD fluorescence intensity histogram profiles at Day 0 (red), Day 30 (blue), and negative control (black) from primary (c) and secondary (f) transplants. Percentage of DiD^high cells in the FoxA2+ cells from primary (d) and secondary (g) transplants. Percentage of DiD^low cells in the FoxA2+ cells from primary (e) and secondary (h) transplants. Data are presented as mean ± SD for each group, n = 3 mice. The asterisks indicate significant difference: two-tailed Student’s unpaired samples t test; ***p = 7.0148E−07 (DiD^high primary transplant D0 vs D30), 7.84512E−06 (DiD^low primary transplant D0 vs D30), 3.32176E−06 (DiD^high secondary transplant D0 vs D30), and 3.46695E−06 (DiD^low secondary transplant D0 vs D30). Complete statistical information is provided in Supplementary Table 1.