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. 2022 May 6;13:2515. doi: 10.1038/s41467-022-30247-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A Tibia sections from FoxA2^CreERT/+; Tomato^fl/+;Tg.col1^GFP mice treated with tamoxifen (Tam) P13-P16, operated via Salter–Harris type 1 (SH1)-like surgery at P18, harvested at 7 days post-op (a). Representative details from the metaphyseal bone in numbered insets (a1–3). Tibia sections from FoxA2^Cre.ERT/+; ZsGreen^f/+;Tg.col10^mcherry mice treated with tamoxifen P13-P16, operated via SH1-like surgery at P18, and harvested 5 days post-op (b). Representative details from the hypertrophic zone in numbered insets (b1-3). Scale bars are 100 µm. B Tibia sections harvested from FoxA2^CreERT/+;Tomato^fl/+ (a) and FoxA2^CreERT/+;DTA^f/+;Tomato^fl/+ (b) mice treated with tamoxifen P13-P16, harvested at P18. Hoechst dye (blue), 5-DTAF (green). Insets, magnified view of the FoxA2+ cells visualized by Tomato fluorescence microscopy (red) (a1, b1). Scale bars 50 µm. Quantification of Tomato+ cells, per 10 µm tibial section of GP tissue, from FoxA2^CreERT/+; Tomato^fl/+ and FoxA2^CreERT/+; DTA^f/+; Tomato^fl/+ mice. Data are presented as mean ± SD of n = 4 mice. The asterisks indicate significant difference: Two-tailed Student’s unpaired samples t test; *p = 0.028571 (FoxA2^CreERT/+; Tomato^fl/+ vs FoxA2^CreERT/+; DTA^f/+; Tomato^fl/+). C Safranin O/Fast Green staining of tibia sections from control DTA^f/f mice (a, b) and FoxA2^CreERT/+; DTA^f/f mice (c, d), treated with tamoxifen P13-P16, operated at P18 and harvested 7 days after injury. Insets, magnified view of the injury callus in FoxA2^CreERT/+; DTA^f/f mice (d1, d2), or DTA^f/f mice (b1, b2). Scale bars are 100 µm. D Image J quantification of GP cartilage regeneration after surgery in FoxA2^CreERT/+; DTA^f/f mice compared with DTA^f/f mice. Percentage (%) cartilage regeneration is calculated by dividing the area of SafraninO+ GP cartilage to the total GP cartilage area (injured plus healthy). Data are presented as mean ± SD, n = 5 mice. The asterisks indicate significant difference: Two-tailed Student’s unpaired-samples t test; **p = 0.007937 (FoxA2^CreERT/+; DTA^f/f vs DTA^f/f). Complete statistical information is provided in Supplementary Table 1.