Fig. 1. wake influences male-male courtship behaviour.

The courtship and chaining indices were defined as the percentages of the 10-min observation period in which the corresponding male-male courtship behaviour was observed. a Locations of the insertions in the different wake mutants (wake³²⁰⁹⁹, wake^GS17103, and wake^NP3168) are indicated by differently coloured arrowheads. b The wake insertion lines have higher male–male courtship behaviour indices than the wild-type (WT) or the revrt^13-1 precise wake³²⁰⁹⁹ transgene excision control lines. n = 18, 18, 19, 17 and 18 (from left to right) for each test, ^****p < 0.0001, two-tailed Mann–Whitney U-test. c  Males homozygous for wake³²⁰⁹⁹ also exhibited a higher level of male-male chaining behaviour than both wake³²⁰⁹⁹ heterozygous (wake³²⁰⁹⁹/+) and revrt^13-1 males. In addition, males homozygous for wake^GS17103 or wake^NP3168 exhibited a very low level of chaining behaviour, which was significantly enhanced in wake³²⁰⁹⁹/wake^GS17103 and wake³²⁰⁹⁹/wake^NP3168 transheterozygotes. n = 6 for each. p < 0.0001, one–way ANOVA. ^****p < 0.0001, post hoc Tukey’s multiple comparisons test. Scatterplots show error bars (± SEM) for all data points. Source data are provided as a Source Data file.