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. 2022 May 6;13:2518. doi: 10.1038/s41467-022-30165-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative images showing the pattern of expression for an RU486-inducible IPC-specific ilp2-GeneSwitch driver; expression patterns in the adult brain (10 days) revealed using UAS-mCD8::GFP are shown as green (n = 6 for each). The neuropil was immunostained using an anti-DLG antibody (magenta). b The cell bodies of IPCs can be observed due to UAS-nls::GFP for the nuclei (green) and were confirmed via anti-Ilp2 antibody staining (magenta) (n = 6 for each). Scale bars, 20 µm. c–d Comparison of genetically manipulated (wake^NP3624>UAS-wake^RNAi, wake-Gal4¹⁵¹⁸⁵>UAS-wake^RNAi, wake^NP3168>UAS-wake^RNAi, wake^NP1350>UAS-wake^RNAi) and RU486-treated (ilp2-GeneSwitch>UAS-wake^RNAi) 5-day-old males with the corresponding controls. Scatter plots include ± SEM for all data points for c the Courtship Index (n = 18 for each) and d Chaining Index (n = 10, 10, 10, 10, 10 and 11; from left to right for test group no. 13–18), p < 0.0001, Kruskal–Wallis test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.005, and ^****p < 0.0001, post hoc Dunn’s multiple comparisons test. Source data are provided as a Source Data file.