Table 3.
Gibberellin, GA19, concentration at 20, 25 and 30 DPA and high pI α-amylase protein content in harvest-ripe (HR) grain of parental varieties and reciprocal F1 hybrid grains
| Line ID | DPA | GA19 concentration ng gDW−1 |
High pI α-amylase ELISA OD at HR |
|---|---|---|---|
| Spica | 20 | 58 ± 10 | |
| Spica | 25 | 122 ± 5 | |
| Spica | 30 | 155 ± 14 | |
| Spica | HR | 0.55 ± 0.02 | |
| Maringa | 20 | nd | |
| Maringa | 25 | nd | |
| Maringa | 30 | nd | |
| Maringa | HR | 0.09 ± 0.004 | |
| Spica/Maringa F1 | 20 | 80 ± 4 | |
| Spica/Maringa F1 | 25 | 83 ± 23 | |
| Spica/Maringa F1 | 30 | 116 ± 40 | |
| Spica/Maringa F1 | HR | 0.075 ± 0.005 | |
| Maringa/Spica F1 | 20 | 45 ± 3 | |
| Maringa/Spica F1 | 25 | 79 ± 6 | |
| Maringa/Spica F1 | 30 | 117 ± 21 | |
| Maringa/Spica F1 | HR | 0.10 ± 0.01 |
The first named parent of the hybrids is the female. Duplicate samples, each consisting of 2–3 spikes and a minimum of 25 F1 grains, were harvested at each sampling time. DW = grain dry weight, standard errors are shown after the ± sign