Fig. 2.

Dose-dependent effects of UCN3 on blood glucose, gastric emptying, glucose-stimulated secretion of insulin and lipolysis in the rat. Response to an oral glucose (2 g/kg) challenge in combination with s.c. injection of either vehicle (control), three different doses of UCN3 (2, 7.5 and 30 nmol/kg), or GLP-1 (7–36amide, 30 nmol/kg). Plots of variables over time are shown, with respective AUCs, for blood glucose (a, b), plasma paracetamol (c, d), plasma insulin (e, f), ∆insulin/∆blood glucose (g, h), plasma NEFA (i, j), plasma TAG (k, l) and plasma glycerol (m, n). AUCs covered −5 min to 45 min (b, d, f, j, l and n) or 5–45 min (h). Baseline sample points taken at −30 and −15 min were in all cases plotted as −5 min and 0 min. Data are shown as means + SEM, n = 8 or 9. *p<0.05, **p<0.01 and ***p<0.001 (two-way ANOVA followed by Tukey’s multiple comparison test, testing groups against vehicle [a, c, e, g, i, k, m] or by one-way ANOVA followed by Tukey’s multiple comparison test [b, d, f, h, j, l, n]). TAG, triacylglycerol. Arrows indicate time point of OGTT and compound administration